产品名称

DNA Ligase from T4-infected Escherichia  coli

别 称

Polydeoxyribonucleotide Synthase,Polynucleotide Ligase, T4 DNA Ligase

含 量

4,000U/mL

CAS  NO.

9015-85-4

分子量

mol wt 68 kDa

MDL  number

MFCD00163508

适用范围

生物试剂

l  理化信息

外 观

缓冲的甘油水溶液

l  生物学信息

生物活性/药理作用

T4 DNA Ligase forms an energy dependent phosphodiester age between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3′ hydroxyl and 5′ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.

应 用

Suitable for:

• Ligation of blunt ended or cohesive DNA fragments

• Ligation of cloning vector and restriction insert fragments

• Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids

• Couple RNA single strands by bridging oligonucleotide adapters

有效单位定义

One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of P32 from pyrophosphate  into ATP as Norit-absorbable material in 20 minutes at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov