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Mutanolysin from Streptomyces globisporus

商品编号: S1216
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国外库存,货期2-4周,具体详情请联系销售人员。

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详细介绍

l  基本信息

产品名称

Mutanolysin from Streptomyces globisporus(变溶菌素来源于球孢链霉菌)

一般描述

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.

效 价

≥4000 units/mg protein (biuret)

CAS NO.

55466-22-3

分子量

23 kDa

适用范围

生物试剂,适用于细胞培养等

l  理化信息

外 观

白色至白色冻干粉末

l  生物学信息

生物活性/药理作用

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(14)-N-acetylglucosamine age of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

应 用

Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci.It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

有效单位定义

One unit will produce a ΔA600 of  0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

样品制备说明

Solutions of mutanolysin can be prepared in 50 mM TES, pH 7.0, with 1 mM MgCl2, at the equivalent of 1 mg/mL. Mutanolysin can also be dissolved in water or TE buffer.

l  存储

存储温度

−20°C

l  注意事项及免责声明

本产品仅用于实验研究,不得作为药物使用,不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov